MIDAS - (DH-ISP) Three-dimensional microscopy and sectional image reconstruction using optical scanning holography

(DH-ISP) Three-dimensional microscopy and sectional image reconstruction using optical scanning holography

MIDAS/ISP: Digital Holography/Article #114194

Title:

(DH-ISP) Three-dimensional microscopy and sectional image reconstruction using optical scanning holography

Authors:

Edmund Y. Lam¹, Xin Zhang¹, Huy Vo², Ting-Chung Poon², Guy Indebetouw²

Institutions:

¹University of Hong Kong, ²Virginia Tech

Publisher:

Optical Society of America

Publication date:

Received 2009-Jul-13

PDF Article:

http://www.opticsinfobase.org/abstract.cfm?uri=ao---

Abstract:

Fast acquisition and high axial resolution are two primary requirements for three-dimensional microscopy. However, they are sometimes conﬂicting: imaging modalities such as confocal imaging can deliver superior resolution at the expense of sequential acquisition at diﬀerent axial planes, which is a time-consuming process. Optical scanning holography (OSH) promises to deliver a good tradeoﬀ between these two goals. With just a single scan, we can capture the entire three-dimensional volume in a digital hologram; the data can then be processed to obtain the individual sections. An accurate modeling of the imaging system is key to devising an appropriate image reconstruction algorithm, especially for real data where random noise and other imaging imperfections must be taken into account. In this paper we demonstrate sectional image reconstruction by applying an inverse imaging sectioning technique to experimental OSH data of biological specimens, and visualizing the sections using the OSA Interactive Science Publishing software.

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